摘要
目的 建立用HPLC-ELSD测定柴胡加龙骨牡蛎汤改善睡眠有效部位中人参皂苷Rg1、人参皂苷Rb1、柴胡皂苷a、柴胡皂苷d、柴胡皂苷B2含量的方法。方法 采用HPLC-ELSD,Diamonsil C18(4.6 mm×250 mm,5 μm)色谱柱,流动相为乙腈-水,梯度洗脱,流速为1.0 mL·min-1,柱温为30 ℃,蒸发光散射检测器漂移管温度为105 ℃,载气流速氮气为2.5 L·min-1。结果 人参皂苷Rg1、人参皂苷Rb1、柴胡皂苷a、柴胡皂苷d、柴胡皂苷B2平均含量分别为0.474%、1.372%、1.554%、0.883%和2.073%,其线性范围分别为1.820~9.10、1.810~9.050、1.130~10.170、0.420~2.100、3.125~15.625 μg,精密度、重复性、加样回收率的RSD均<3.0%。结论 该方法快捷,准确,重复性好,能同时测定柴胡加龙骨牡蛎汤改善睡眠有效部位中5种成分的含量,为柴胡加龙骨牡蛎汤有效部位质量标准的建立奠定了一定科学基础。
Abstract
OBJECTIVE To establish a method for content determination of ginsenoside Rg1, ginsenoside Rb1, saikosaponin a, saikosaponin d, and saikosaponin B2 in 70% ethanol elution effective fraction of Chaihu plus Longgu Muli decoction by HPLC-ELSD. METHODS A Diamonsil C18 column(4.6 mm×250 mm, 5 μm) was used as the stationary phase and the mobile phase consisted of acetonitrile and water. Gradient elution was carried out at the flow rate of 1.0 mL·min-1. The column temperature was maitained at 30 ℃. The ELSD detector was operated at 105 ℃ with nebulizing gas at the optimum flow rate of 2.5 L·min-1. RESULTS The average contents of ginsenoside Rg1, ginsenoside Rb1, saikosaponin a, saikosaponin d, and saikosaponin B2 were 0.474%, 1.372%, 1.554%, 0.883%, and 2.073%, respectively. The calibration curves were linear in the ranges of 1.820-9.10, 1.810-9.050, 1.130-10.170, 0.420-2.100, and 3.125-15.625 μg, respectively. The RSDs of precision, reproducibility and recovery were all less than 3.0%. CONCLUSION The method is rapid, simple, reliable and accurate, and has been successfully used to the quantification of five components in 70% ethanol elution effective fraction of Chaihu plus Longgu Muli decoction, which can provide a basis for the quality evaluation of Chaihu plus Longgu Muli decoction.
关键词
高效液相色谱蒸发光散射检测器 /
柴胡加龙骨牡蛎汤 /
皂苷 /
睡眠障碍
{{custom_keyword}} /
Key words
HPLC-ELSD /
Chaihu plus Longgu Muli decoction /
saponin /
sleep disorder
{{custom_keyword}} /
许丹丹, 杜纳纳, 耿一玄, 黄莉莉.
HPLC-ELSD测定柴胡加龙骨牡蛎汤改善睡眠有效部分中5种皂苷类成分的含量[J]. 中国药学杂志, 2017, 52(10): 861-864 https://doi.org/10.11669/cpj.2017.10.012
XU Dan-dan, DU Na-na, GENG Yi-xuan, HUANG Li-li.
Determination of Five Saponins in 70% Ethanol Elution Effective Fraction of Chaihu Plus Longgu Muli Decoction by HPLC-ELSD[J]. Chinese Pharmaceutical Journal, 2017, 52(10): 861-864 https://doi.org/10.11669/cpj.2017.10.012
中图分类号：
R284
{{custom_clc.code}}
({{custom_clc.text}})
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
[1] DU N N,YU S,HUANG L L. Studies on effective parts of Chaihu Jia Longgu Muli Decoction for improving sleep of perimenopausal female mice [J]. Tradit Chin Drug Res Clin Pharmacol(中国新药与临床药理),2014,25(5):556-559.
[2] SUN B,HAO H Q,ZHENG K J,et al. Preliminary study on mechanism of saikosaponins in regulating sleep rhythmical electrical activities in cat[J]. J Tianjin Med Univ(天津医科大学学报),2000,6(3):274-276.
[3] LI Y F,YAN X K,LI T L,et al. Experimental study on the effects of saikosaponin C in freeze-dry powder of Si-Ni-San on sleep time in mice[J]. Chin J Clin Pharmacol (中国临床药理学杂志),2011,27(8):601-603.
[4] WU H F,ZHU C H,GUO J Y. Effect of ginsenoside Rg1 on behaviors and hippocampal amino acids in depressive-like rats[J]. China J Chin Mater Med(中国中药杂志),2012,37(20):3117-3221.
[5] DONG J Y,WANG J B,FANG J,et al. Effects of ginsenosides Rb1 on learning and memory and expression of somatostatin in sleep deprivation rats[J]. J Zhejiang Univ(Med Sci)(浙江大学学报:医学版),2013,42(2):197-204.
[6] YANG Q Y,LI X Y,LIU G L. Research progress in pharmacological activities of ginsenoside Rb1[J]. Chin Pharm J(中国药学杂志),2013,48(15):1233-1237.
[7] ZHANG H,YAN L,LIN L F,et al. Determination of seven index components in Xiaochaihu Granules by HPLC [J]. Drugs Clin,2014,29(2):162-165.
[8] SHAO Y,PU M B. Content determination of saikosaponin a and d in Xiaochaihu Granules by HPLC[J]. Chin J Tradit Med Sci Technol(中国中医药科技),2010,17(4):334-335.
[9] WEI P. Determination of 3 ginsenosides in Shenzhu Tablet Simultaneously by HPLC [J]. World J Integr Tradit West Med(世界中西医结合杂志),2007,2:215-216.
{{custom_fnGroup.title_cn}}
脚注
{{custom_fn.content}}
基金
黑龙江省普通高等学校青年学术骨干支持计划资助项目(1254G054);黑龙江省博士后科研启动金二等资助项目(LBH-Q13161);黑龙江中医药大学校科研基金资助项目(2012bs01); 黑龙江中医药大学“优秀创新人才支持计划”资助项目(2012RCD02)
{{custom_fund}}
PDF(1152 KB)
